Plasmid

Part:BBa_K4716008:Design

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


pSB1C3-J23119-CsgA-Perpa-mefp5-mSandy


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2049
    Illegal NheI site found at 2085
    Illegal NheI site found at 2108
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2049
    Illegal BamHI site found at 3556
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2049
    Illegal XbaI site found at 2064
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use this backbone (pSB1C3) is because it is a classic backbone to express target gene in E. coli strain and it is a high copy BioBrick assembly plasmid with a chloramphenicol resistance marker.


Source

The backbone of this composite part is pSB1C3 from BBa_J179000.

References